Journal: Journal of molecular modeling

Article Title: Molecular Modeling in the Age of Clinical Genomics, The Enterprise of the Next Generation

doi: 10.1007/s00894-017-3258-3

Figure Lengend Snippet: Known structures of XIAP Structures highlighted in yellow were used for modeling the complete XIAP structure ( Figure 2B ) and those in red used to model XIAP-protein interactions ( ).

Article Snippet: 4EC4 , X-ray , 3.3 , A/B/C/D/E/F/G/J/K/L , 241–356 , BIR3 , SMAC MIMETIC (0O6) , .

Techniques: Binding Assay

Journal: Journal of carcinogenesis & mutagenesis

Article Title: Inhibitor of Apoptosis Proteins: Promising Targets for Cancer Therapy

doi: 10.4172/2157-2518.S14-004

Figure Lengend Snippet: Pre-clinical data where IAP inhibition sensitised to anti-cancer therapies.

Article Snippet: , Smac mimetc (BV6) , Radiotherapy , [ ] .

Techniques: Inhibition, shRNA

Journal: PLoS ONE

Article Title: EGFR-Targeted TRAIL and a Smac Mimetic Synergize to Overcome Apoptosis Resistance in KRAS Mutant Colorectal Cancer Cells

doi: 10.1371/journal.pone.0107165

Figure Lengend Snippet: (a–d) Caco-2tet Ras G12V cells were seeded into 3D cultures in the presence of doxycycline. (a) Three days later, cultures were left untreated or treated with 5 µM SM83 or 20 µM Z-VAD for 1 h prior to addition of 1 nM Db αEGFR -scTRAIL. Viability was measured 24 h later by MTT assay and normalized to the untreated control (n = 3). (b) 24 h after treatment, cells were fixed and stained for DNA strand breaks. Tunel-positive cells were counted (n = 2). (c) Three days post seeding, cultures were left untreated (ut) or treated with 5 µM SM83. Cells were recovered from the cultures 24 h later and lysates were analyzed by immunoblotting. Shown is one representative blot of two independent experiments. Tubulin was detected as a loading control. (d) Quantification of Western blots from (c). Protein levels were normalized to the corresponding tubulin control; levels in the untreated cultures were set as 1 (n = 2). (e, f) HCT-116 and LoVo cells were grown in 3D cultures for six days, and then fixed and stained for F-actin and DNA (DAPI) (scale bar: 20 µm) (top panels). Three days post seeding, cultures were left untreated or pretreated with 5 µM SM83 prior to addition of 0.05 nM Db αEGFR -scTRAIL. Viability was measured 24 h later by MTT assay and normalized to the untreated control (bottom panels) (n = 3).

Article Snippet: In the presence of doxycycline, these cells showed increased resistance to Db αEGFR -scTRAIL, associated with the elevated expression of the anti-apoptotic proteins cIAP2, Bcl-xL and Flip S . Co-treatment of cells with the Smac mimetic SM83 restored the Db αEGFR -scTRAIL-induced apoptotic response.

Techniques: MTT Assay, Control, Staining, TUNEL Assay, Western Blot